The caterpillar feeds on various cruciferous species.
Euchloe ausonia inhabits dry-warm, usually semi-open habitats such as bushy rocky slopes, clear olive groves, forest edges or dry ruderal terrain.
Euchloe ausonia occurs in two generations from late February to June or early July. The maximum is in April and May and the second generation is only partial. The caterpillar lives from April to June or July and is quite striking on the host plant. The pupa aestivates and then hibernates.
Euchloe ausonia occurs in Italy north to Tuscany and from the southern Balkans and Turkey across the Aegean Sea to Western Asia.
The very similar Euchloe crameri is the Southwestern European vicariant of Euchloe ausonia.